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FUJIFILM nylon fiber column t
Nylon Fiber Column T, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nylon+fiber+column+t/nylon+fiber+column+t/pmc03224891-73-10-15
Average 90 stars, based on 1 article reviews
nylon fiber column t - by Bioz Stars, 2026-10
90/100 stars

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MTT Assay:

Article Title: Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo.
Article Snippet: Contents lists available at SciVerse ScienceDirect European Journal of Pharmacology j ourna l homepage: www.e lsev ie r .com/ locate /e jphar Immunopharmacology and Inflammation Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo Fengyang Chen a, Yang Ni a,c, Yiping Ye a,⁎, Hongxiang Sun b,⁎⁎, Xiaoyu Li a, Shifang Xu a a Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China b Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China c Zhejiang Hospital of Chinese Medicine, Hangzhou 310006, China ⁎ Corresponding author at: Institute of Materia M Medical Sciences, Tianmushan Road 182, Hangzho Tel./fax: +86 571 8821 5624.. ⁎⁎ Corresponding author at: Key Laboratory of Anima culture, College of Animal Sciences, Zhejiang Unive Hangzhou, Zhejiang, 310058, China.. Tel./fax: +86 571 8 E-mail addresses: yeyiping2005@163.com (Y. Ye), 0014-2999/$ – see front matter © 2012 Elsevier B.V. All doi:10.1016/j.ejphar.2012.04.012 a b s t r a c t a r t i c l e i n f o Article history: Received 29 January 2012 Received in revised form 28 March 2012 Accepted 5 April 2012 Available online 20 April 2012 Keywords: Stephanthraniline A Immunosuppressive T cell Stephanotis mucronata Stephanthraniline A (STA) isolated from the stems of Stephanotis mucronata (Blanco) Merr. was evaluated for their suppression on T cells' immune responses in vitro and in vivo.

Functional Assay:

Article Title: Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo.
Article Snippet: Contents lists available at SciVerse ScienceDirect European Journal of Pharmacology j ourna l homepage: www.e lsev ie r .com/ locate /e jphar Immunopharmacology and Inflammation Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo Fengyang Chen a, Yang Ni a,c, Yiping Ye a,⁎, Hongxiang Sun b,⁎⁎, Xiaoyu Li a, Shifang Xu a a Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China b Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China c Zhejiang Hospital of Chinese Medicine, Hangzhou 310006, China ⁎ Corresponding author at: Institute of Materia M Medical Sciences, Tianmushan Road 182, Hangzho Tel./fax: +86 571 8821 5624.. ⁎⁎ Corresponding author at: Key Laboratory of Anima culture, College of Animal Sciences, Zhejiang Unive Hangzhou, Zhejiang, 310058, China.. Tel./fax: +86 571 8 E-mail addresses: yeyiping2005@163.com (Y. Ye), 0014-2999/$ – see front matter © 2012 Elsevier B.V. All doi:10.1016/j.ejphar.2012.04.012 a b s t r a c t a r t i c l e i n f o Article history: Received 29 January 2012 Received in revised form 28 March 2012 Accepted 5 April 2012 Available online 20 April 2012 Keywords: Stephanthraniline A Immunosuppressive T cell Stephanotis mucronata Stephanthraniline A (STA) isolated from the stems of Stephanotis mucronata (Blanco) Merr. was evaluated for their suppression on T cells' immune responses in vitro and in vivo.

Purification:

Article Title: Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo.
Article Snippet: Contents lists available at SciVerse ScienceDirect European Journal of Pharmacology j ourna l homepage: www.e lsev ie r .com/ locate /e jphar Immunopharmacology and Inflammation Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo Fengyang Chen a, Yang Ni a,c, Yiping Ye a,⁎, Hongxiang Sun b,⁎⁎, Xiaoyu Li a, Shifang Xu a a Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China b Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China c Zhejiang Hospital of Chinese Medicine, Hangzhou 310006, China ⁎ Corresponding author at: Institute of Materia M Medical Sciences, Tianmushan Road 182, Hangzho Tel./fax: +86 571 8821 5624.. ⁎⁎ Corresponding author at: Key Laboratory of Anima culture, College of Animal Sciences, Zhejiang Unive Hangzhou, Zhejiang, 310058, China.. Tel./fax: +86 571 8 E-mail addresses: yeyiping2005@163.com (Y. Ye), 0014-2999/$ – see front matter © 2012 Elsevier B.V. All doi:10.1016/j.ejphar.2012.04.012 a b s t r a c t a r t i c l e i n f o Article history: Received 29 January 2012 Received in revised form 28 March 2012 Accepted 5 April 2012 Available online 20 April 2012 Keywords: Stephanthraniline A Immunosuppressive T cell Stephanotis mucronata Stephanthraniline A (STA) isolated from the stems of Stephanotis mucronata (Blanco) Merr. was evaluated for their suppression on T cells' immune responses in vitro and in vivo.

Article Title: XBP1s repression regulates Kupffer cell polarization leading to immune suppressive effects protecting liver allograft in rats.
Article Snippet: .. They then purified the cells using a Nylon Fiber Column T (Wako, Ltd., W. Zhang et al. International Immunopharmacology 91 (2021) 107294 Japan). ..

Article Title: MyD88-silenced dendritic cells induce T-cell hyporesponsiveness and promote Th2 polarization in vivo.
Article Snippet: Background aims.. Previous studies have determined that the absence of MyD88 enhances the tolerogenicity of dendritic cells (DCs), suggesting that inhibiting innate immunity may be a potential strategy to facilitate the induction of transplant tolerance by DCs.. However, the underlying mechanism remains unclear.

Enzyme-linked Immunosorbent Assay:

Article Title: Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo.
Article Snippet: Contents lists available at SciVerse ScienceDirect European Journal of Pharmacology j ourna l homepage: www.e lsev ie r .com/ locate /e jphar Immunopharmacology and Inflammation Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo Fengyang Chen a, Yang Ni a,c, Yiping Ye a,⁎, Hongxiang Sun b,⁎⁎, Xiaoyu Li a, Shifang Xu a a Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China b Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China c Zhejiang Hospital of Chinese Medicine, Hangzhou 310006, China ⁎ Corresponding author at: Institute of Materia M Medical Sciences, Tianmushan Road 182, Hangzho Tel./fax: +86 571 8821 5624.. ⁎⁎ Corresponding author at: Key Laboratory of Anima culture, College of Animal Sciences, Zhejiang Unive Hangzhou, Zhejiang, 310058, China.. Tel./fax: +86 571 8 E-mail addresses: yeyiping2005@163.com (Y. Ye), 0014-2999/$ – see front matter © 2012 Elsevier B.V. All doi:10.1016/j.ejphar.2012.04.012 a b s t r a c t a r t i c l e i n f o Article history: Received 29 January 2012 Received in revised form 28 March 2012 Accepted 5 April 2012 Available online 20 April 2012 Keywords: Stephanthraniline A Immunosuppressive T cell Stephanotis mucronata Stephanthraniline A (STA) isolated from the stems of Stephanotis mucronata (Blanco) Merr. was evaluated for their suppression on T cells' immune responses in vitro and in vivo.

Reverse Transcription:

Article Title: Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo.
Article Snippet: Contents lists available at SciVerse ScienceDirect European Journal of Pharmacology j ourna l homepage: www.e lsev ie r .com/ locate /e jphar Immunopharmacology and Inflammation Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo Fengyang Chen a, Yang Ni a,c, Yiping Ye a,⁎, Hongxiang Sun b,⁎⁎, Xiaoyu Li a, Shifang Xu a a Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China b Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China c Zhejiang Hospital of Chinese Medicine, Hangzhou 310006, China ⁎ Corresponding author at: Institute of Materia M Medical Sciences, Tianmushan Road 182, Hangzho Tel./fax: +86 571 8821 5624.. ⁎⁎ Corresponding author at: Key Laboratory of Anima culture, College of Animal Sciences, Zhejiang Unive Hangzhou, Zhejiang, 310058, China.. Tel./fax: +86 571 8 E-mail addresses: yeyiping2005@163.com (Y. Ye), 0014-2999/$ – see front matter © 2012 Elsevier B.V. All doi:10.1016/j.ejphar.2012.04.012 a b s t r a c t a r t i c l e i n f o Article history: Received 29 January 2012 Received in revised form 28 March 2012 Accepted 5 April 2012 Available online 20 April 2012 Keywords: Stephanthraniline A Immunosuppressive T cell Stephanotis mucronata Stephanthraniline A (STA) isolated from the stems of Stephanotis mucronata (Blanco) Merr. was evaluated for their suppression on T cells' immune responses in vitro and in vivo.

Activation Assay:

Article Title: Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo.
Article Snippet: Contents lists available at SciVerse ScienceDirect European Journal of Pharmacology j ourna l homepage: www.e lsev ie r .com/ locate /e jphar Immunopharmacology and Inflammation Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo Fengyang Chen a, Yang Ni a,c, Yiping Ye a,⁎, Hongxiang Sun b,⁎⁎, Xiaoyu Li a, Shifang Xu a a Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China b Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China c Zhejiang Hospital of Chinese Medicine, Hangzhou 310006, China ⁎ Corresponding author at: Institute of Materia M Medical Sciences, Tianmushan Road 182, Hangzho Tel./fax: +86 571 8821 5624.. ⁎⁎ Corresponding author at: Key Laboratory of Anima culture, College of Animal Sciences, Zhejiang Unive Hangzhou, Zhejiang, 310058, China.. Tel./fax: +86 571 8 E-mail addresses: yeyiping2005@163.com (Y. Ye), 0014-2999/$ – see front matter © 2012 Elsevier B.V. All doi:10.1016/j.ejphar.2012.04.012 a b s t r a c t a r t i c l e i n f o Article history: Received 29 January 2012 Received in revised form 28 March 2012 Accepted 5 April 2012 Available online 20 April 2012 Keywords: Stephanthraniline A Immunosuppressive T cell Stephanotis mucronata Stephanthraniline A (STA) isolated from the stems of Stephanotis mucronata (Blanco) Merr. was evaluated for their suppression on T cells' immune responses in vitro and in vivo.

Transduction:

Article Title: Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo.
Article Snippet: Contents lists available at SciVerse ScienceDirect European Journal of Pharmacology j ourna l homepage: www.e lsev ie r .com/ locate /e jphar Immunopharmacology and Inflammation Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo Fengyang Chen a, Yang Ni a,c, Yiping Ye a,⁎, Hongxiang Sun b,⁎⁎, Xiaoyu Li a, Shifang Xu a a Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China b Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China c Zhejiang Hospital of Chinese Medicine, Hangzhou 310006, China ⁎ Corresponding author at: Institute of Materia M Medical Sciences, Tianmushan Road 182, Hangzho Tel./fax: +86 571 8821 5624.. ⁎⁎ Corresponding author at: Key Laboratory of Anima culture, College of Animal Sciences, Zhejiang Unive Hangzhou, Zhejiang, 310058, China.. Tel./fax: +86 571 8 E-mail addresses: yeyiping2005@163.com (Y. Ye), 0014-2999/$ – see front matter © 2012 Elsevier B.V. All doi:10.1016/j.ejphar.2012.04.012 a b s t r a c t a r t i c l e i n f o Article history: Received 29 January 2012 Received in revised form 28 March 2012 Accepted 5 April 2012 Available online 20 April 2012 Keywords: Stephanthraniline A Immunosuppressive T cell Stephanotis mucronata Stephanthraniline A (STA) isolated from the stems of Stephanotis mucronata (Blanco) Merr. was evaluated for their suppression on T cells' immune responses in vitro and in vivo.

Polymerase Chain Reaction:

Article Title: Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo.
Article Snippet: Contents lists available at SciVerse ScienceDirect European Journal of Pharmacology j ourna l homepage: www.e lsev ie r .com/ locate /e jphar Immunopharmacology and Inflammation Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo Fengyang Chen a, Yang Ni a,c, Yiping Ye a,⁎, Hongxiang Sun b,⁎⁎, Xiaoyu Li a, Shifang Xu a a Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China b Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China c Zhejiang Hospital of Chinese Medicine, Hangzhou 310006, China ⁎ Corresponding author at: Institute of Materia M Medical Sciences, Tianmushan Road 182, Hangzho Tel./fax: +86 571 8821 5624.. ⁎⁎ Corresponding author at: Key Laboratory of Anima culture, College of Animal Sciences, Zhejiang Unive Hangzhou, Zhejiang, 310058, China.. Tel./fax: +86 571 8 E-mail addresses: yeyiping2005@163.com (Y. Ye), 0014-2999/$ – see front matter © 2012 Elsevier B.V. All doi:10.1016/j.ejphar.2012.04.012 a b s t r a c t a r t i c l e i n f o Article history: Received 29 January 2012 Received in revised form 28 March 2012 Accepted 5 April 2012 Available online 20 April 2012 Keywords: Stephanthraniline A Immunosuppressive T cell Stephanotis mucronata Stephanthraniline A (STA) isolated from the stems of Stephanotis mucronata (Blanco) Merr. was evaluated for their suppression on T cells' immune responses in vitro and in vivo.

Staining:

Article Title: Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo.
Article Snippet: Contents lists available at SciVerse ScienceDirect European Journal of Pharmacology j ourna l homepage: www.e lsev ie r .com/ locate /e jphar Immunopharmacology and Inflammation Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo Fengyang Chen a, Yang Ni a,c, Yiping Ye a,⁎, Hongxiang Sun b,⁎⁎, Xiaoyu Li a, Shifang Xu a a Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China b Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China c Zhejiang Hospital of Chinese Medicine, Hangzhou 310006, China ⁎ Corresponding author at: Institute of Materia M Medical Sciences, Tianmushan Road 182, Hangzho Tel./fax: +86 571 8821 5624.. ⁎⁎ Corresponding author at: Key Laboratory of Anima culture, College of Animal Sciences, Zhejiang Unive Hangzhou, Zhejiang, 310058, China.. Tel./fax: +86 571 8 E-mail addresses: yeyiping2005@163.com (Y. Ye), 0014-2999/$ – see front matter © 2012 Elsevier B.V. All doi:10.1016/j.ejphar.2012.04.012 a b s t r a c t a r t i c l e i n f o Article history: Received 29 January 2012 Received in revised form 28 March 2012 Accepted 5 April 2012 Available online 20 April 2012 Keywords: Stephanthraniline A Immunosuppressive T cell Stephanotis mucronata Stephanthraniline A (STA) isolated from the stems of Stephanotis mucronata (Blanco) Merr. was evaluated for their suppression on T cells' immune responses in vitro and in vivo.

Isolation:

Article Title: Anti-tumor effects of a human VEGFR-2-based DNA vaccine in mouse models
Article Snippet: .. T lymphocytes were isolated from single-cell suspensions with using a Nylon Fiber Column T (L-Type, WAKO) to use as CTL effector cells; MS1 murine endothelial cells, which express mFlk-1[ ], were used as target cells. ..

Article Title: Mechanisms for enhanced antitumor immune responses induced by irradiated hepatocellular carcinoma cells engineered to express hepatitis B virus X protein
Article Snippet: .. Spleens of 8- to 12-week-old mice were aseptically removed, naïve T lymphocytes were isolated from single-cell suspensions with Nylon Fiber Column T (Wako Chemicals USA, Inc., Richmond, VA, USA). ..

Article Title: MyD88-silenced dendritic cells induce T-cell hyporesponsiveness and promote Th2 polarization in vivo.
Article Snippet: Background aims.. Previous studies have determined that the absence of MyD88 enhances the tolerogenicity of dendritic cells (DCs), suggesting that inhibiting innate immunity may be a potential strategy to facilitate the induction of transplant tolerance by DCs.. However, the underlying mechanism remains unclear.

Article Title: Endoplasmic reticulum stress and its regulator XBP-1 contributes to dendritic cell maturation and activation induced by high mobility group box-1 protein.
Article Snippet: High mobility group box-1 protein (HMGB1) had been proved to induce maturation and activation of dendritic cell (DC), however, the endogenous changes and mechanisms underlying are unknown.. Since endoplasmic reticulum stress (ERS) activates an adaptive unfolded protein response (UPR) that facilitates cellular survival and repair, we hypothesized that HMGB1 may regulate the function of DC by modulating ERS.. In our study, HMGB1 stimulation induced significant ERS responses in DCs in a timeand dose-dependent manner, demonstrated by the up-regulation of a number of ERS markers.

Article Title: Simian virus 40 inhibits differentiation and maturation of rhesus macaque DC-SIGN + -dendritic cells
Article Snippet: Monocytes were separated from lymphocyte by glass adherence, and proliferated by using RPMI 1640 medium (GIBCO) containing 10% FBS, 20 mM HEPES, 1 mM sodium pyruvate, 2 mM L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, 500 ng/ml recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF, R&D Systems) at 37°C, 5% CO 2 , 100% relative humidity, and induced to differentiate toward IDC by using 10 ng/ml recombinant human interleukin-4 (rhIL-4, R&D Systems). .. T-lymphocytes were isolated from cell suspensions consisting of T cells and B cells with Nylon Fiber Column T (Wako) and cultured in RPMI 1640 medium supplemented with 1 ng/ml recombinant human interleukin-2 (rhIL-2, R&D Systems). ..

Single Cell:

Article Title: Mechanisms for enhanced antitumor immune responses induced by irradiated hepatocellular carcinoma cells engineered to express hepatitis B virus X protein
Article Snippet: .. Spleens of 8- to 12-week-old mice were aseptically removed, naïve T lymphocytes were isolated from single-cell suspensions with Nylon Fiber Column T (Wako Chemicals USA, Inc., Richmond, VA, USA). ..

Lysis:

Article Title: MyD88-silenced dendritic cells induce T-cell hyporesponsiveness and promote Th2 polarization in vivo.
Article Snippet: Background aims.. Previous studies have determined that the absence of MyD88 enhances the tolerogenicity of dendritic cells (DCs), suggesting that inhibiting innate immunity may be a potential strategy to facilitate the induction of transplant tolerance by DCs.. However, the underlying mechanism remains unclear.

Gradient Centrifugation:

Article Title: MyD88-silenced dendritic cells induce T-cell hyporesponsiveness and promote Th2 polarization in vivo.
Article Snippet: Background aims.. Previous studies have determined that the absence of MyD88 enhances the tolerogenicity of dendritic cells (DCs), suggesting that inhibiting innate immunity may be a potential strategy to facilitate the induction of transplant tolerance by DCs.. However, the underlying mechanism remains unclear.

Modification:

Article Title: MyD88-silenced dendritic cells induce T-cell hyporesponsiveness and promote Th2 polarization in vivo.
Article Snippet: Background aims.. Previous studies have determined that the absence of MyD88 enhances the tolerogenicity of dendritic cells (DCs), suggesting that inhibiting innate immunity may be a potential strategy to facilitate the induction of transplant tolerance by DCs.. However, the underlying mechanism remains unclear.

Cell Culture:

Article Title: Simian virus 40 inhibits differentiation and maturation of rhesus macaque DC-SIGN + -dendritic cells
Article Snippet: Monocytes were separated from lymphocyte by glass adherence, and proliferated by using RPMI 1640 medium (GIBCO) containing 10% FBS, 20 mM HEPES, 1 mM sodium pyruvate, 2 mM L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, 500 ng/ml recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF, R&D Systems) at 37°C, 5% CO 2 , 100% relative humidity, and induced to differentiate toward IDC by using 10 ng/ml recombinant human interleukin-4 (rhIL-4, R&D Systems). .. T-lymphocytes were isolated from cell suspensions consisting of T cells and B cells with Nylon Fiber Column T (Wako) and cultured in RPMI 1640 medium supplemented with 1 ng/ml recombinant human interleukin-2 (rhIL-2, R&D Systems). ..

Recombinant:

Article Title: Simian virus 40 inhibits differentiation and maturation of rhesus macaque DC-SIGN + -dendritic cells
Article Snippet: Monocytes were separated from lymphocyte by glass adherence, and proliferated by using RPMI 1640 medium (GIBCO) containing 10% FBS, 20 mM HEPES, 1 mM sodium pyruvate, 2 mM L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, 500 ng/ml recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF, R&D Systems) at 37°C, 5% CO 2 , 100% relative humidity, and induced to differentiate toward IDC by using 10 ng/ml recombinant human interleukin-4 (rhIL-4, R&D Systems). .. T-lymphocytes were isolated from cell suspensions consisting of T cells and B cells with Nylon Fiber Column T (Wako) and cultured in RPMI 1640 medium supplemented with 1 ng/ml recombinant human interleukin-2 (rhIL-2, R&D Systems). ..

Incubation:

Article Title: Permanent acceptance of mouse cardiac allografts with CD40 siRNA to induce regulatory myeloid cells by use of a novel polysaccharide siRNA delivery system.
Article Snippet: Leukocyte isolation Spleens were removed, ground through a 70 μm cell strainer and red blood lysed (Sigma, St Louis, MO, USA; RBC lysis buffer). .. Cells were loaded onto the Nylon Fiber Column T (Wako, Osaka, Japan), and incubated at 37 ° C and 5% CO2 for 60min. ..



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